摘要目的 探讨富含亮氨酸重复序列免疫球蛋白样蛋白1(LRIG1)和表皮生长因子受体(EGFR)在食管鳞癌中的表达与意义.方法 应用逆转录聚合酶链反应(RT-PCR)方法检测46例食管癌肿瘤组织LRIG1和EGFR基因的表达情况,并与46例癌旁正常组织进行比较分析.结果 食管癌组织中LRIG1 mRNA的表达水平(0.35±0.04)低于相应癌旁正常组织(0.67±0.07),P﹤0.05;食管癌组织中EGFR mRNA的表达水平(2.23±0.25)高于相应癌旁正常组织(1.88±0.21),P﹤0.05;食管癌组织、相应癌旁正常组织中LRIG1 mRNA和EGFR mRNA的表达水平呈负相关,差异有统计学意义(r=-0.662,P﹤0.05).结论 LRIG1 mRNA在食管癌组织中存在低表达,提示其有可能具有抑癌作用.EGFR mRNA在食管癌组织中存在高表达,且LRIG1 mRNA和EGFR mRNA二者之间呈负相关,提示LRIG1 mRNA表达下降可能导致EGFR 活化,从而促进食管癌的发生发展.

abstractsObjective To investigate the expression of LRIG1 and epithelial growth factor receptor(EGFR)in esophageal carcinoma. Methods The expression of LRIG1 and EGFR were detected by RT-PCR in 46 cases of esophageal carcinoma and comparing with 46 cases normal para-cancer tissues. Results The expression level of LRIG1 mRNA in esophageal carcinoma tissues(0.35±0.04) was lower than that in in esophageal carcinoma tissues(2.23±0.25) was higher than that in normal expression level of LRIG1 mRNA and EGFR mRNA in esophageal carcinoma tissues and normal para-cancer tissues,there were significant differences between them(r=-0.662,P<0.05). Conclusions The expression level of LRIG1 mRNA is lower in esophageal carcinoma tissues than that in normal para-cancer tissues,which reveals that LRIG1 gene may be an anti-oncogene. The high expression level of EGFR mRNA in esophageal carcinoma tissues and negative correlation between the expression level of LRIG1 mRNA and EGFR mRNA in human esophageal tissues, may reveal that the lower expression of LRIG1 mRNA lead to the reduction of inhibition signal to EGFR and active it, results in the progression of esophageal carcinoma.