摘要目的 观察非小细胞肺癌( NSCLC)标本中基质金属蛋白酶2(MMP 2)和MMP 9及血管内皮生长因子(VEGF)的表达,以探讨其在NSCLC中表达的意义,为临床的诊断、治疗和预后的判断提供参考.方法 用SP免疫组化技术检测MMP 2、MMP 9、VEGF在77例肺癌组织中的表达.结果 肺癌组织中MMP 2、MMP 9主要表达于肿瘤细胞的胞浆,在癌旁交界区组织也有表达,肿瘤组织的过表达率显著高于交界区(P＜0.05),MMP 2、VEGF在肿瘤组织的过表达率与淋巴结转移状态、临床分期与原发肿瘤大小有关(P＜0.05).结论 MMP 2、MMP 9、VEGF都参与了非小细胞癌发生、发展过程,MMP 2、VEGF在其中可能起到协同促进作用,MMP 2、VEGF联合分析可能更有助于评估NSCLC患者的预后.

abstractsObjective To investigate the expression and clinic pathological features of MMP2,MMP9 and VEGF in non - small - cell lung carcinoma,and provide reference for diagnosis,treatment and prognosis predictment of the disease.Methods MMP 2 and MMP 9,VEGF expressions were detected in 77 cases of non - small - cell lung carcinoma tissues and their adjacent tissues and 24 cases of normal lung tissues by steptavidinperoxdase immunohistochemical technique.Results The immunoreactivities of MMP 2 and MMP 9,VEGF were mainly shown in cytoplasma of tumor cells.The overexpressions of MMP 2 and MMP 9,VEGF in carcinoma tissues were significantly higher than in adjacent tissues ( P ＜ 0.05).The overexpression of MMP 2 and VEGF in the cancer tissues were related to clinical stages,lymph node metastasis status and the size of primary cancer ( P ＜ 0.05 ).There was a trend that the survival rate of these patients with overexpression of MMP 2 and MMP 9,VEGF was lower than that with non - overexpression,but there was no statistical significance.The survival rate of the group with both MMP 2 and MMP 9,VEGF overexpressions was lower than with non - overexpression ( P ＜ 0.05 ).Conclusions MMP 2,MMP 9 and VEGF all take part in the progress of non - small - cell lung carcinoma.We postulate that MMP 2 can cooperate with VEGF in the processes.To investigate the over - expressions of both MMP 2 and MMP 9 in non - small - cell lung carcinoma tissues may be more helpful for estimating the prognosis.