摘要目的 研究慢性肝病血清中可溶性Ⅰ型补体受体(sCR1)的浓度变化和透明质酸的含量变化,并分析二者的相关性,以探讨其临床意义.方法 采用双抗体夹心ELISA法测定血清中sCR1浓度,采用放射免疫法测定血清透明质酸含量.结果 对慢性肝患者群及正常人群进行研究表明,不同肝病患者血清sCR1水平不同程度的升高,慢性肝病血清sCR1含量(0.79±0.20)显著高于正常人群(0.51±0.24) (P ＜0.01);并且22例肝硬化患者血清sCR1含量显著高于慢性肝患者群(P＜0.01);血清sCR1与透明质酸(HA)二者密切相关(r=0.83).结论 慢性肝病血清sCR1的变化、透明质酸含量的变化与肝脏损伤严重程度密切相关,可为慢性肝病患者的早期、快速、准确诊断提供相应的理论依据.

abstractsObjective To study the relationship between the changes of serum levels of soluble complement receptor type 1 (sCR1) and HA in patients with chronic liver disease,and investigate the significance of sCR1 and HA.Methods Enzyme-linked immunosorbent assay (ELISA) was used in detecting the serum levels of sCR1,and radioimmuassay was used in detecting the serum levels of HA.Results The results showed that the serum levels of sCR1 in patients with chronic liver disease (0.79 ±0.20,t =2.1) were significantly higher(P ＜0.01) than those in healthy individuals (0.51 ±0.24).The serum levels of sCR1 in 22 hepatocirrhosis patients were significantly higher than those in patients with chronic liver disease (P ＜ 0.01) and correlation with HA (r =0.83).Conclusions The serum levels of sCR1 and HA accumulates in patients with chronic liver disease are closely related with the severity of liver injury,and it can provide a theoretical basis for early,rapid and accurate diagnosis of patients with chronic liver disease.