摘要OBJECTIVE:To investigate the efficacy of Gouqizi(Fructus Lycii)seed oil(FLSO)on D-gal induced inflammation in testis of rats in vitro and in vivo.METHODS:In aging Sertoli cells(TM4 cells)induced by D-galactose(D-gal),the expression of upregulated aging-related proteins.The number of cells counted by cell counting kit(CCK)-8 assay showed a high number of cells disposed with FLSO at 50,100 and 150 μg/mL compared to that for the aging model.In vivo,male Sprague-Dawley rats(n=50,8-week-old,230-255 g)were randomly categorized into control,aging model,and FLSO(low-,medium-,and high-dose)groups.The expression of nuclear factor-KB(NF-κB)and its upstream factors[Janus kinase 1(JAK1)and signal transducerand activator of transcription 1(STAT1)]was detected by Western blot and immunofluorescence,related inflammatory factors quantified by enzyme-linked immunosorbent assay.Evaluation of testicular tissue by Johnsen score,the spermatogenic function was explored.RESULTS:The expression of interleukin-1β(IL-1β)(P＜0.05),IL-6(P＜0.001),and tumor necrosis factor a(TNF-a)(P＜0.05)was decreased significantly,while that of heme oxygenase-1(HO-1)(P＜0.001)and IL-10(P＜0.05)was increased in cells disposed with FLSO 100 μg/mL.FLSO inhibited the expression of NF-κB and declined p-p65/p65(P＜0.01),as detected by Western blotting.In vivo,the levels in serum of IL-1β(P＜0.001),IL-6(P＜0.05),and TNF-a(P＜0.01)declined while IL-10(P＜0.05)was upregulated post-FLSO treatment.In addition,the expression of JAK-1 and STAT1 increased significantly in testicular tissue of rats treated with FLSO as compared to the aging model of rats(P＜0.001),while the expression of NF-κB(P＜0.001)declined in the testis in the FLSO group,as assessed by immunofluorescence.The levels of inhibor B and testosterone in serum both increased(P＜0.05).CONCLUSIONS:In conclusion,this study determined the protective effects of FLSO to tolerate inflammatory injury in the testis,indicating that FLSO alleviates inflammation via JAK-1/STAT1/NF-κB pathway.