摘要OBJECTIVE:To investigate the outcome of Chinese water-soluble propolis(WSP)on the inflammatory response and oxidative stress(OS)of colonic mucosa in rats with ulcerative colitis.METHODS:Dextran sulfate sodium(DSS)was employed to establish the ucerative colitis(UC)rat model.Forty-eight male rats were arbitrarily separated into six groups,namely control,UC,low-dose water-soluble propolis(L-WSP),medium-dose water-soluble propolis(M-WSP),high-dose water-soluble propolis(H-WSP),and sulfasalazine(Sulfa).In this study,we adopted a method of pre-administration and reconstruction of the model that assessed the water-soluble propolis mediated protection against DSS-induced UC rats.Moreover,we examined the body weight(BW),disease activity index(DAI),bloody stool,colon length,and intestinal mucosal injury index of rats.In addition,using enzyme linked immunosorbent assays,we assessed indicators,such as,colonic myeloperoxidase(MPO),interleukin-6(IL-6),interleukin-9(IL-9),tumor necrosis factor-α(TNF-α),superoxide dismutase(SOD),malondialdehyde,and glutathione peroxidase(GSH-Px)levels.RESULTS:The pro-inflammatory cytokine expression,as well as OS,was increased in the model rats.However,upon WSP intervention,both pro-inflammatory cytokine levels and OS reduced dramatically,and the therapeutic effect was dose-dependent.CONCLUSION:WSP downregulates OS by enhancing the function of endogenous antioxidant enzymes like SOD and GSH-Px,that inhibit neutrophil activity,as well as diminish pro-inflammatory cytokines like TNF-α,IL-6,and IL-9,along with mechanisms that attenuate intestinal inflammation in UC rat model.