检索历史 清除

摘要Part One:The establishment and evaluation of chronicunpredictable mild stress depression model<br>　　Objective:To explore the validity and reliability of the dynamicchronic unpredictable mild stress model of depression.<br>　　Methods:The body weight、fluid consumption test and Morris watermaze test were measured at the beginning and perweek.<br>　　Result:On 2nd week,the body weight and consumption ofpreference for sucrose solutions of depressive rat were significantlyreduced while the average latent period of Morris water maze test wassignificantly increased on 3rd week,which changed dynamically with thestress.<br>　　Conclusion:The rat model of depression is an ideal model whichcan be established successfully by exposing to an unpredictable sequenceof stressors and the behavioral changes can maintain a long time.<br>　　Part Two:Dynamic study of magnetic resonance spectrum abouthippocampus of rat depressive model<br>　　Object:To explore the dynamic change feature and regularity ofvarious metabolisms about hippocampus by the rat depressive model.<br>　　Methods:Thedepressive group was breed solitarily and exposed to chronicunpredictable mild stress in 8 weeks.<br>　　Result:The values of Glx/Cr of the left hippocampus of the depressivegroup were significantly reduced on 3th week,andkeep the same level on 4-8th week;The values ofGlx/Cr of the right hippocampus of the depressive group weresignificantly reduced on 4th week,and keep the samelevel on 5-8th week.<br>　　Conclusion:The metabolic about hippocampus of rat depressive modelbecome abnormal and changes dynamically,this abnormal metabolicchange maybe constitute the nervous biochemical basis of depression.<br>　　Part Three:The research of sensibility about prefrontal cortex andhippocampus of rat depressive model affected by the inflammatoryfactor in 1H-MRS<br>　　Objective:To explore the sensibility of the encephalic region affectedby the inflammatory factor and the role of the inflammatory factor in thedevelopment of the depression.<br>　　Methods:The control group and themodel group for 4 weeks,Using analyzing technology 1H-MRS to detecting prefrontalcortex and hippocampus of each group and detecting the levels of IL-6、IL-2、IL-10 in CSF at the beginning and 4 weeks later.<br>　　Results:The values of Glx/Cr and NAA/Cr in bilateral prefrontal cortex were reduced,The value of Cho/ Cr has nosignificant difference;The values of Glx/Cr in left hippocampus reducedsignificantly,The value of NAA/Cr and Cho/Cr was changed,The values of Glx/Cr、NAA/Cr were reduced and the value of Cho/Cr was increased inright hippocampus;the depressive group has the significantly higher levels of IL-6、IL-2 inCSF.And there was correlation apparently between the values of Glx/Cr in left hippocampus and the levels of IL-6、IL-2.<br>　　Conclusion:The left hippocampus has the more sensibility effected bythe inflammatory factor than other encephalic regions,the high levels ofthe IL-6、IL-2 in CSF and the sensibility of the left hippocampus maybeconstitute the physiopathology basis of the depression.<br>　　Part Four:The study of Magnetic Resonance Spectrum aboutsensitive brain structure of the first episode depression<br>　　Object:To explore the abnormality of the brain's metabolism about thehippocampus,anterior cingulate and thalamus of first episode depressivepatients.<br>　　Methods:Using Proton magnetic resonance spectroscopy and analyzingtechnology 1H-MRS to detect multiple metabolic indices of thehippocampus,anterior cingulate and thalamus of 20 patients and 20healthy persons,including NAA,Cho,MI,Cr.<br>　　Result:The values of MI/Cr of the bilateral thalamus of the depressivepatients group decreased;The values of NAA/Cr of the bilateral hippocampus of thedepressive patients group were lower,the Cho/Cr were higher.<br>　　Conclusion:The metabolic abnormalities of hippocampus,anteriorcingulate and thalamus maybe constitute the nervous biochemical basis offirst episode depression.