摘要The present invention relates to a steroid-cationic lipid compound as represented by formula (I) and the use thereof. In particular, the prepared LNP can efficiently and stably deliver a bioactive substance to a target cell or organ, and particularly, mRNA LNP prepared therefrom has a relatively good stability and intracellular transfection efficiency, and can cause a relatively high specific antibody response and cellular immune response in an animal.