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摘要Disclosed is a method for preparing Au-Ru nanoenzymes, with steps as: 1) dissolving HAuCl4 and ruthenium metal salt in solvent to obtain metal salt solution; 2) adding NAC and TA to the metal salt solution, mixing them uniformly to obtain mixture; 3) adding NaBH4 aqueous solution to the mixture, stirring to react at room temperature, then continuing to stir to react at 50° C.˜70° C.; after the reaction is finished, performing centrifuging, and the precipitate is lyophilized to obtain Aux2014;Ru nanoenzymes. The present invention further discloses a method for preparing ATBMzyme nanoenzymes, specifically: adding EDC and NHS to Aux2014;Ru nanoenzymes solution, stirring to react, then adding brain natriuretic peptide to the reaction system, continuing to stir to react; after the reaction is finished, performing centrifuging, and the precipitate is lyophilized to obtain ATBMzyme nanoenzymes.