摘要目的 分析结肠癌组织中p53、Ki67的表达水平及临床意义,为病情及预后评估提供参考依据.方法 选取2017年1月至2018年7月入住本院结肠癌根治术患者手术切除标本67例,通过免疫组化法测定结肠癌组织以及癌旁黏膜组织中p53、Ki67的表达情况,分析p53、Ki67阳性表达与临床病理特征的关系;并比较p53、Ki67阳性与阴性表达患者之间生存情况.结果 p53、Ki67在结肠癌组织中的阳性率分别为71.64％(48/67)、82.09％(55/67),均明显高于癌旁黏膜组织,差异均有统计学意义(均P<0.05);Ⅰ期+Ⅱ期、高分化、无淋巴结转移以及未达浆膜层患者p53阳性表达率均低于Ⅲ期+Ⅳ期、中低分化、有淋巴结转移、侵及浆膜层,差异均有统计学意义(均P<0.05);随访至2017年7月,p53阳性患者3年存活率为64.58％,明显低于p53阴性患者,差异有统计学意义(P<0.05).结论 结肠癌组织中p53、Ki67均呈明显高表达,Ki67与细胞增殖有关,p53可作为评估结肠癌患者病情及预后的重要指标.

abstractsObjective To analyze the expression of p53 and Ki67 in colon cancer and the clinical significance, and to provide some references for the evaluation of the disease and prognosis. Methods The expressions of p53 and Ki67 in pericancer mucosa and colon cancer were detected by immunohistochemistry. The relationship between the positive expressions of p53 and Ki67 and clinicopathological features was analyzed. The survival rates of the patients positive and negative in p53 and Ki67 expressions were compared. Results The positive rates of p53 and Ki67 in colon cancer were 71.64％(48/67) and 82.09％(55/67), which were significantly higher than those of the pericancer mucosa, with statistical differences (P < 0.05). The positive rates of p53 in stage III and stage IV, medium and poorly differentiated, lymphaticmetastasis, invasion to the serosa were significantly higher than those in Stage I and II, high differentiated, non-lymphaticmetastasis, and non invasion to the serosa, with statistical differences (P < 0.05). The patients were followed up until July, 2017; the 3 year survival rate of the p53 positive patients was 64.58％, which was significantly lower than that of the p53 negative patients, with a statistical difference (P < 0.05). Conclusion There are high expressions of p53 and Ki67 in colon cancer. Ki67 is involved in cell proliferation. P53 can be used as an important indicator to evaluate the conditions and prognosis of colon cancer patients.