摘要目的:对二十年来噬菌体研究的发展情况、发展趋势等问题进行分析并展望未来研究。方法:基于过去二十年发表文献数据使用Citespace分析并进行可视化。结果:噬菌体研究的热点从以分子生物学相关的基础研究逐渐向以噬菌体展示技术、噬菌体治疗和规律间隔成簇短回文重复序列及其关联蛋白系统工具研究为代表的应用研究过渡。目前噬菌体研究的发展主要由发达国家引领，且研究主要集中在目前流行的耐药细菌。结论:以应用为导向进一步加深机制研究以及拓宽噬菌体的研究领域，应成为未来噬菌体研究的重点。

abstractsObjective:To analyze the trend of phage research in the past 20 years and give prospect to future research.Methods:Data visualization was performed based on the analysis of literature published in the past two decades, using Citespace.Results:Hotspots of phage research shifted gradually from basic researches related to molecular biology to application researches represented by phage display technology, phage therapy, clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein genetic tools, etc. At present, the development of phage research is mainly led by developed countries and the research is mainly focused on the current prevalence of multi-drug resistant bacteria.Conclusion:The focus of phage research in future should be deepening the mechanism research and broadening the phage research with application as the orientation.