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目的 检测胃癌组织和癌旁组织中microRNA (miRNA)的差异表达.方法 收集经病理确诊为胃癌进行外科手术切除的25例患者的胃癌组织及距离胃癌病灶5 cm以上的癌旁组织,选取其中7例标本提取总RNA,应用Illumina miRNA芯片检测胃癌和癌旁组织中差异表达的miRNA.应用定量实时PCR技术验证25例胃癌和癌旁组织标本中差异表达的miRNA,并分析25例患者的临床病理与胃癌组织中miRNA差异表达的关联.结果 Illumina miRNA芯片检测显示,HS-138,HS-153,HS-157在胃癌组织中表达下调,miR-181a,miR-21,miR-21*,miR-27a,miR-584,miR-93在胃癌组织中表达上调.定量实时PCR显示,与癌旁组织比较,胃癌组织中miR-181a表达上调(56.848±135.551比3.950±12.101,P＜0.05),而miR-584在胃癌和癌旁组织中的表达差异无统计学意义(P＞0.05).胃癌组织miR-181a表达与患者胃癌淋巴结转移及年龄有关联(rp=0.462、0.414,P=0.009、0.023),与性别和病理分型无关联(P=0.220、0.106).结论 应用miRNA芯片技术和荧光定量PCR技术发现了胃癌组织中差异表达的miRNA.

Objective To study the difference in microRNAs (miRNAs) expressions between gastric cancer tissues and adjacent normal tissues.Methods Snap frozen tissues of gastric carcinoma confirmed histologically and normal gastric tissues located ＞ 5 cm away from the tumor margin were collected during surgical intervention from 25 patients.Seven samples from the 25 patients were extracted for total RNA which was then used to detect differential miRNAs expression in gastric cancer tissues and adjacent normal tissues on Illumina microarray platform.Quantitative real-time PCR analysis was performed in 25patients to compare the expressions of differential miRNAs between cancer tissues and normal gastric tissues.And the correlation between clinicopathological features and differential miRNAs in 25 patients was analyzed.Results Illumina microarray showed down-regulation of HS-138,HS-153 and HS-157,and up-regulation of miR-181a,miR-21,miR-21*,miR-27a,miR-584 and miR-93 in gastric cancer tissues.Compared with normal gastric tissues,a statistically differential up- regulation of miR- 18 la (56.848 ±135.551 vs 3.950±12.101,P＜0.05) but not of miR-584 (P＞0.05) was noted in gastric cancer tissue by quantitative real-time PCR.The expression of miR- 181a in gastric cancer tissue was correlated with lymph node metastasis (rp=0.462,P=0.009) and patient age (rp=0.414,P=0.023),but was not with gender (P=0.220) and pathological types (P=0.106).Conclusion Differentially expressed miRNAs in gastric carcinoma tissues may be detectable with miRNAs microarray and fluorescent quantitative PCR.