摘要目的 探讨蛋白质分子生物标记物SOX9在胃癌组织中的表达及其与临床病理特征及预后的关系.方法 回顾性分析2004-2006年浙江省人民医院收治的112例胃癌患者的临床资料,通过免疫组织化学(IHC)法检测112例原发胃癌组织及70例癌旁非肿瘤组织中SOX9蛋白表达情况.结果 70例癌旁非肿瘤胃黏膜上皮中仅有5例(7.1％)为SOX9低表达;112例胃癌组织中94例(83.9％)出现不同程度的表达,其中SOX9高表达51例(45.5％).胃癌组织中SOX9的表达与患者性别、年龄、肿瘤部位、肿瘤大小及其分化程度无关(P＞0.05),而与肿瘤的Lauren分型(P＜0.05)、浸润深度(P＜0.01)、淋巴结转移(P＜0.05)、远处转移(P＜0.05)及肿瘤TNM分期(P＜0.05)有关.Kaplan-Meier生存分析显示,SOX9高表达患者的5年生存率显著低于SOX9低表达者(29.4％比49.2％,P=0.031).多因素Cox回归分析显示,分化程度(P=0.046)、浸润深度(P=0.001)和远处转移(P＜0.01)是影响胃癌患者预后的独立因素,而SOX9的高表达(P=0.948)不能作为影响胃癌预后的独立因素.结论 胃癌组织中SOX9蛋白的表达与胃癌生长发展和侵袭转移有关并对胃癌患者预后有影响,但不是影响预后的独立因素.

abstractsObjective To investigate the association of SOX9 expression and clinicopathologic factors and prognosis of gastric cancer.Methods A retrospective cohort study including 112 gastric cancer patients admitted to the Zhejiang Provincial People's Hospital from 2004 to 2006 was performed.Immunohistochemical analysis was used to evaluate the expression of SOX9 in the 112 specimens of gastric cancer tissues and 70 non-cancerous tissues adjacent to the tumor.Results Low expression of SOX9 was seen in 5 (7.1％) tissues out of 70 non-cancerous tissues adjacent to the tumor.A total of 94 (83.9％) patients had varying expression of SOX9,of whom 51 (45.4％) had overexpression.Univariate analysis demonstrated that the expression of SOX9 was significantly associated with Lauren classification (P＜0.05),tumor invasion (P＜0.01),lymph node metastasis (P＜0.05),distant metastasis (P＜0.05) and tumor stage (P＜0.05),however there was no significant association between SOX9 expression and sex,age,histological type,histology differentiation or tumor size.Kaplan-Meier analysis showed that the 5-year survival rate of patients with SOX9 over-expression was significantly lower than that of patients with low expression (29.4％ vs.49.2％,P=0.031).Multivariate Cox regression analysis showed that histology differentiation (P=0.046),tumor invasion (P=0.001),and distant metastasis (P＜0.01) were independent prognostic factors for gastric cancer,however the over-expression of SOX9 was not significant(P=0.948).Conclusions The expression SOX9 is associated with the growth,invasion,and metastasis of gastric cancer,as well as the prognosis.However,SOX9 expression is not an independent factor for the prognosis in patients with gastric cancer.