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Change and significance of nuclear factor-κB in adriamycin induced cardiomyopathy in rats

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Background This study aimed at investigating the change and significance of nuclear factor-κB (NF-κB) in cardiomyopathy induced by adriamycin (ADR) in rats.Methods Sixty male Wistar rats were randomly divided into three groups: control, ADR and ADR+pyrrolidine dithiocarbamate (PDTC) groups. After 30-day experiment, myocardial histopathological observation was performed. Location and distribution of NF-κB p50 was examined by immunohistochemical assay. Expression of NF-κB p50 protein was examined by immunobolt assay. Electrophoretic Mobility Shift Assay examined activity of NF-κB; Myocardium p53 gene expression was examined by RT-PCR analysis. Results The myocardial lesions of rats were less pronounced in ADR +PDTC group than in ADR group. Compared with control group, there were many myocardium nucleuses, which expressed NF-κB p50 and distribute under epicardium. Expression of NF-κB p50 protein in nucleus increased significantly in ADR group. The NF-κB binding activity increased significantly in ADR group. Myocardium expressions of p53 mRNA increased in ADR group. Conclusions The NF-κB binding activity increased significantly in cardiomyopathy induced by ADR in rats. Moreover, NF-κB plays an important role in causing degeneration of myocardial tissue and regulating expression of related-apoptosis genes.

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作者单位: Department of Hemopathy, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China [1] Harbin First Hospital,Harbin Medical University Harbin, China [2] Keshan Disease Institute,Harbin Medical University Harbin, China [3]
期刊: 《中华医学杂志(英文版)》2005年118卷2期 111-115页 SCIMEDLINEISTICCSCDBP
分类号: R5
栏目名称: ORIGINAL ARTICLES
发布时间: 2005-03-24
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