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Testin on Atherosclerosis in Rabbits

摘要:

Background:The expression of TES,a novel tumor suppressor gene,is found to be down-regulated in the left anterior descending aorta of patients with coronary artery disease (CAD) compared with non-CAD subjects.This study aimed to investigate the expression of TES during the development of atherosclerosis in rabbits.Methods:Thirty-two New Zealand rabbits were randomly divided into a normal diet (ND) and high-fat diet (HFD) groups.Body weight and serum lipid levels were measured at 0,4,and 12 weeks after diet treatment.The degree of atherosclerosis in thoracic aortas was analyzed by histological examinations.The expression of Testin in the tissue samples was inspected via immunohistochemical and immunofluorescence confocal microscopy.Real time-polymerase chain reaction and Western blot analysis were performed to evaluate the expression of TES/Testin at mRNA and protein levels in the aortic tissues.Results:After 12 weeks postenrollment,rabbits in HFD group had a higher level of serum lipids and atherosclerotic plaque compared to ND group (P < 0.05).Testin expression was detected at high levels in the endothelium and a weak expression on the subendothelium area.The expression of TES mRNA was markedly reduced by 10-fold in the aortic tissues in the HFD group compared with the ND group (P =0.015),and the protein level was also significantly decreased in the HFD group (P < 0.05).Conclusions:Reduced TES/Testin expression is associated with the development of atherosclerosis,implicating a potentially important role in the pathogenesis of atherosclerosis.

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作者单位: Department of Cardiology,Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease(Key Lab-TIC),Tianjin Institute of Cardiology,Second Hospital of Tianjin Medical University,Tianjin 300211,China [1] Collaborative Innovation Center of Tianjin for Medical Epigenetics,Department of Physiology and Pathophysiology,Tianjin Medical University,Tianjin 300070,China [2] Department of Cardiology,Binzhou People's Hospital,Binzhou,Shandong 256600,China [3]
期刊: 《中华医学杂志(英文版)》2015年128卷12期 1662-1665页 SCIMEDLINEISTICCSCDBP
栏目名称: Original Articles
DOI: 10.4103/0366-6999.158370
发布时间: 2015-07-15
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