检索历史 清除

目的：通过研究雷公藤甲素（triptolide，TP）对人口腔鳞状细胞癌细胞株（human oral cancer cell，HB）增殖和PTEN基因（人第10号染色体缺失的磷酸酶及张力蛋白同源的基因）表达的影响，探讨TP抗HB的机制，以期为口腔鳞状细胞癌的药物治疗提供依据。方法用含0（空白对照组）、1.25、2.50、5.00和10.00μg/L的TP溶液培养HB 24、48和72 h，甲基噻唑基四唑法检测细胞生长抑制率，流式细胞仪检测细胞周期变化，反转录PCR（reverse transcription-PCR，RT-PCR）检测PTEN mRNA表达，蛋白质印迹法检测PTEN蛋白表达。结果1.25、2.50、5.00和10.00μg/L的TP处理72 h后，HB生长抑制率分别为（26.92±0.14）%、（38.67±0.11）%、（72.62±0.89）%和（90.42±0.28）%；PTEN mRNA表达量分别为（3.59±0.21）%、（5.27±0.40）%、（7.18±0.44）%和（9.16±0.50）%。PTEN蛋白A值分别为0.135±0.007、0.410±0.020、0.447±0.017和0.884±0.066。与空白对照组相比，10.00μg/L的TP处理48 h后G1期细胞由（58.78±0.98）%增加到（84.13±0.47）%，S期细胞则由（25.40±0.43）%降低到（9.41±0.73）%。结论 TP对HB增殖有抑制作用，TP可影响细胞周期，可能通过下调PTEN表达发挥抗癌作用。

Objective To investigate the effect of triptolide on human oral cancer cell (HB) proliferation and phosphates and tensin homologue deleted on chromosome ten gene (PTEN) mRNA expression in oral cancer. Methods The cancer cells were cultured in the medium containing triptolide of different concentrations for 24, 48 and 72 h. Methyl thiazolyl tetrazolium (MTT) method was used to test the rate of growth inhibition of cancer cells, flow cytometer to detect the change of cell cycle and reveres transcription-PCR (RT-PCR) to examine the expression of PTEN mRNA. The expression of PTEN protein was examined by Western blotting. Results The rate of growth inhibition was (26.92 ± 0.14)%, (38.67 ± 0.11)%, (72.62 ± 0.89)% and (90.42 ± 0.28)%, respectively. The corresponding expression of PTEN mRNA was (3.59±0.21)%, (5.27±0.40)%, (7.18±0.44)% and (9.16±0.50)%, respectively and the corresponding A value of PTEN protein was 0.135±0.007, 0.410±0.020, 0.447±0.017 and 0.884±0.066, respectively. The proportion of G1 phase cells increased from (58.78±0.98)%to (84.13±0.47)%, but the proportion of S phase cells decreased from (25.40±0.43)%to (9.41±0.73)%. Conclusions The triptolide not only had inhibitory effect on the HB proliferation, but also affected the cell cycle.