摘要目的 研究尸体供肠的获取与保存方法.方法 采用原位灌洗、整块切取的方法自6具尸体获取供肠,Euro-Collins液保存,光镜和电镜下观察供肠的组织学变化,其中2例供肠分别移植至2例短肠综合征患者.结果 6例尸体供肠完整切取的时间为(10.8±1.4)min,热缺血时间为(5.6±1.2)min;光镜及电镜检查证实保存10 h内的供肠组织损伤轻微;第1例移植的小肠运动和吸收功能逐渐恢复,后因肠道和肺部感染死亡,第2例患者恢复无脂饮食.结论 该法实用、有效,所获小肠可用于临床移植.

abstractsObjective To investigate the procurement and preservation of small intestinal graft from cadaveric donor.Methods The donor was perfused in sim and the graft including small intestine,pancreas,kidneys and liver was procured en bloc.The graft was preserved by cold Euro-Collins solution.Structure and ultrastructure of the graft were observed.Two grafts were transplanted into 2 patients with short bowel syndrome.Results The 6 grafts were procured from cadaveric donors according to the above method.The grafts were procured within(10.8±1.4)min and the warm ischemia time was(5.6±1.2)min.The results of light and electron microscopical observation showed that tissue injury was slight following the graft preserved within 10 h.In the first patient,absorptive and motive function of the graft was restored,and the second patient had oral diet without fat.Conclusions This method of procurement and preservation of small intestinal graft was suitable to cadaveric donors.