摘要目的 观察OSI-027对肝内胆管细胞癌细胞株RBE和HCCC-9810增殖的影响和OSI-027与顺铂联用在肝内胆管细胞癌治疗中的作用及其机制.方法 分别用OSI-027、顺铂、OSI-027和顺铂联用处理肝内胆管细胞癌细胞株RBE、HCCC-9810.细胞计数试剂盒(CCK-8)实验检测细胞活力,EdU实验检测细胞增殖,流式细胞术检测细胞凋亡.对照组为不做任何处理的RBE、HCCC-9810细胞.结果 OSI-027可以显著抑制RBE和HCCC-9810的细胞活力和增殖,表现为显著的时间和浓度依赖性.单独使用OSI-027不能诱导RBE和HCCC-9810凋亡,而单独使用顺铂能够诱导RBE和HCCC-9810凋亡[RBE:(6.56±1.27)％,HCCC-9810:(9.25±1.49)％].OSI-027与顺铂联用显著增强顺铂诱导的RBE和HCCC-9810细胞凋亡[RBE:(16.78±2.23)％,HCCC-9810:(21.47±2.17)％].结论 OSI-027可以抑制肝内胆管细胞癌的增殖,其与顺铂联用后可增强顺铂对肝内胆管细胞癌的杀伤作用.

abstractsObjective To investigate the effect and mechanism of OSI-027 combined with cisplatin on proliferation and apoptosis of human intrahepatic cholangiocarcinoma (ICC) cell lines RBE and HCCC-9810.Methods RBE and HCCC-9810 cells were treated with OSI-027,cisplatin,or OSI-027 plus cisplatin,respectively.Cell counting kit-8 (CCK-8) kit was used to test cell viability.The proliferation and apotosis of ICC cell lines were examined by Ethynyl deoxyuridine (EdU) assay and flow-cytomery,separately.The cells not given treatment served as control group.Results OSI-027 significantly inhibited the proliferation of RBE and HCCC-9810 cells in a concerntration-and time-dependent manner.Cisplatin,but not OSI-027,induced apoptosis of RBE and HCCC-9810 cells [RBE:(6.56 ± 1.27) ％,HCCC-9810:(9.25 ± 1.49) ％].When OSI-027 combined with cisplatin was given,apopotosis of ICC cells occurred apparently [RBE:(16.78 ± 2.23) ％,HCCC-9810:(21.47 ±2.17) ％].The combination of cisplatin and OSI-027 displayed more efficient in vitro.Conclusion OSI-027 suppressed proliferation of ICC cell lines and enhanced apotosis induced by cisplatin in vitro.