摘要目的 探讨细胞周期蛋白依赖性激酶(CDK)2在单纯疱疹病毒(HSV)复制中的作用. 方法 以能够诱导表达CDK2显性负突变体致使CDK2功能缺陷的人结肠癌细胞株HT29Tet-Ondn-CDK2为材料,用免疫共沉淀及放射自显影法测定HSV感染后不同时间CDK2活性,并进行HSV的增殖动力学分析. 结果 HSV感染6h后CDK2活性被诱导,9h达到最大;一步生长曲线结果表明在CDK2活性被诱导前HSV处于静止状态,CDK2活性被诱导后HSV即进入快速的生长复制阶段. 结论 CDK2是HSV复制所必需的,HSV通过诱导宿主细胞CDK2活性而促进本身的复制.

abstractsObjective To explore the effect of cyclindependent kinase(CDK)2 on the replication of herpes simplex virus(HSV).Methods Dominant-negative CDK2 expressing cell line HT29Tet-On dn-CDl(2 was used as host cells to determine the role of CDK2 in HSV rephcation.HT29Tet-0n dn-CDK2 cells were infected with HSV.The activity of CDK2 Was determined by the immunoprecipitate and autoradiograph before and after HSV infection.The growth curve of HSV Was conducted.Results The CDK2 activity was induced after infection for 6 h and reached the hishest level at 9 h.HSV replicated rapidly and productively after the CDK2 was induced.Coatiusion CDK2 is required for the replication of HSV,and HSV replicates in host cells by inducing the CDK2 activity.